Melan-A / MART-1 Antibody

ProSci
Product Code: PSI-33-646
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-646-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Further Information

Additional Names:
MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
Application Note:
Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1ug/10^6 cells

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT

The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.
Background:
MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.
NCBI Gene ID #:
2315
NCBI Official Name:
melan-A
NCBI Official Symbol:
MLANA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Immunology
Swissprot #:
Q16655
User NOte:
Optimal dilutions for each application to be determined by the researcher