MAML2 Antibody [MMLP2-1]

Flow Cytometry: 0.5-1ug/10^6 cells

IF: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML2 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Mastermind-like 2, also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, it interacts with the ankyrin repeats of the Notch proteins (1-4). This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

A human recombinant protein was used as the immunogen for this MAML2 antibody.

84441

Heat shock protein

Hsp-16

Caenorhabditis elegans

Liquid

Protein G affinity chromatography

Stem Cell

P06582

Optimal dilutions for each application to be determined by the researcher