MCM7 Antibody [SPM379]

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ProSci
Product Code: PSI-33-735
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-735-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM379
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

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IHC testing of FFPE human tonsil with MCM7 antibody (clone SPM379). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human tonsil with MCM7 antibody (clone SPM379). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Documents

Further Information

Additional Names:
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Application Note:
IF: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

WB: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Titering of the MCM7 antibody may be required for optimal performance.
Background:
MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human full length recombinant protein was used as the immunogen for the MCM7 antibody.
NCBI Gene ID #:
4176
NCBI Official Name:
minichromosome maintenance complex component 7
NCBI Official Symbol:
MCM7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
P33993
User NOte:
Optimal dilutions for each application to be determined by the researcher