MCM7 Antibody [MCM7/1468]

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ProSci
Product Code: PSI-33-847
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-847-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: MCM7/1468
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MCM7 antibody (clone MCM7/1468). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MCM7 antibody (clone MCM7/1468). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Additional Names:
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Application Note:
Immunofluorescence: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the MCM7 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.
NCBI Gene ID #:
4176
NCBI Official Name:
minichromosome maintenance complex component 7
NCBI Official Symbol:
MCM7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
P33993
User NOte:
Optimal dilutions for each application to be determined by the researcher