Napsin A Antibody [NAPSA/1239]

Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Western blot: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Napsin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.

9476

Napsin-A

NAPSA

Homo sapiens

Liquid

Protein G affinity chromatography

Cell Cycle

O96009

Optimal dilutions for each application to be determined by the researcher