Neurofilament Antibody [NF421]

NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

Flow Cytometry: 0.5-1 ug/million cells

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2)

Prediluted format : incubate for 30 min at RT (3)

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Human recombinant Neurofilament protein was used as the immunogen. (1)

4744

neurofilament, heavy polypeptide

NEFH

Homo sapiens

Liquid

Protein G purified Neurofilament antibody

Neuroscience, Signal Transduction, Stem Cell

P12036

Optimal dilutions for each application to be determined by the researcher