ODC Antibody [ORN255-1]

ProSci
Product Code: PSI-33-884
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-884-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ORN255-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 3
Western blot testing of ODC antibody and placenta lysate.
2 / 3
3 / 3

Western blot testing of ODC antibody and placenta lysate.

Further Information

Additional Names:
Ornithine decarboxylase, ODC, ODC1
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the ODC antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Ornithine Decarboxylase (ODC) is the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [RefSeq]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for this ODC antibody.
NCBI Gene ID #:
4953
NCBI Official Name:
ornithine decarboxylase 1
NCBI Official Symbol:
ODC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity,Signal Transduction
Swissprot #:
P11926
User NOte:
Optimal dilutions for each application to be determined by the researcher