Eosinophil Peroxidase Antibody [AHE-1]
| Code | Size | Price |
|---|
| PSI-33-228-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: AHE-1
Regulatory Status: RUO
Target Species: Human
Application: Immunofluorescence (IF)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
EPX, EPO, EPP, EPX-PEN
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
IF: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Background:
Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human eosinophils from a patient with hypereosinophilic syndrome were used as the immunogen for this Eosinophil peroxidase antibody.
NCBI Gene ID #:
8288
NCBI Official Name:
eosinophil peroxidase
NCBI Official Symbol:
EPX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified monoclonal antibody
Research Area:
Signal Transduction
Swissprot #:
P11678
User NOte:
Optimal dilutions for each application to be determined by the researcher