Gamma Catenin Antibody [CTNG/1664]
| Code | Size | Price |
|---|
| PSI-33-610-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTNG/1664
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, JUP, CTNNG, DP3
Application Note:
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IHC (FFPE): 0.1-0.2 ug/ml
The stated application concentrations are suggested starting amounts. Titration of the Gamma Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IHC (FFPE): 0.1-0.2 ug/ml
The stated application concentrations are suggested starting amounts. Titration of the Gamma Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
Plakoglobin, also known as junction plakoglobin or gamma Catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to beta-catenin. It is a cytoplasmic component of desmosomes and adherens junctions structures located within intercalated discs of cardiac muscle that function to anchor sarcomeres and join adjacent cells in cardiac muscle. Mutations in Gamma Catenin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.
Gamma Catenin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. It links cadherins to the actin cytoskeleton. Gamma Catenin binds to conserved regions of desmoglein and desmocollin at intracellular catenin-binding sites to assemble desmosomes. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the Gamma Catenin antibody.
NCBI Gene ID #:
3728
NCBI Official Name:
junction plakoglobin
NCBI Official Symbol:
JUP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P14923
User NOte:
Optimal dilutions for each application to be determined by the researcher