PSAP Antibody [ACCP/1338]

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ProSci
Product Code: PSI-33-762
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-762-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ACCP/1338
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of human prostate carcinoma with PSAP antibody (clone ACCP/1338). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human prostate carcinoma with PSAP antibody (clone ACCP/1338). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT

Titering of the PSAP antibody may be required for optimal performance.
Background:
Prostate specific acid phosphatase (PSAP) is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant full length protein was used as the immunogen for this PSAP antibody.
NCBI Gene ID #:
55
NCBI Official Name:
acid phosphatase, prostate
NCBI Official Symbol:
ACPP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle
Swissprot #:
P15309
User NOte:
Optimal dilutions for each application to be determined by the researcher