Phosphotyrosine Antibody [PY20]
| Code | Size | Price |
|---|
| PSI-33-259-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PY20
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.
1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Background:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
NCBI Gene ID #:
Not Applicable
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity, Signal Transduction, Neuroscience, Cancer
User NOte:
Optimal dilutions for each application to be determined by the researcher