Phosphotyrosine Antibody [PY20] (PE)
| Code | Size | Price |
|---|
| PSI-98-518-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: [PY20]
Regulatory Status: RUO
Shipping:
blue ice
Storage:
Store vial at 2-8˚C
Documents
Further Information
Additional Names:
pTyr20
Application Note:
Phosphotyrosine Antibody [PY20] for use in flow cytometry assays.
Background:
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.
Buffer:
Supplied in PBS/NaN3 and a stabilizing agent
Concentration:
0.1 mg/mL
Conjugate:
PE
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Phosphotyrosine conjugated to carrier proteins
Physical State:
Liquid
Research Area:
Obesity,Signal Transduction,Neuroscience,Cancer
SPECIFICITY:
Phosphotyrosine
User NOte:
Optimal dilutions for each application to be determined by the researcher.