HLA-ABC Antibody [246-B8.E7]

ProSci
Product Code: PSI-33-388
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-388-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 246-B8.E7
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Non-Human Primates
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41kDa.
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Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41kDa.

Further Information

Application Note:
Western blot: 1-2 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Optimal dilution of the HLA-ABC antibody should be determined by the researcher.
Background:
Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P01889, P30443, P30499
User NOte:
Optimal dilutions for each application to be determined by the researcher