Integrin beta 3 Antibody [Y2/51]

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ProSci
Product Code: PSI-33-627
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-627-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: Y2/51
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human spleen tissue with CD61 antibody (clone Y2/51). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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IHC testing of FFPE human spleen tissue with CD61 antibody (clone Y2/51). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

Documents

Further Information

Additional Names:
Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD61, ITGB3, GP3A
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Titration of the CD61 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain). It associates with the alphaV-chain (CD51) to form vitronectin receptor, or with the alphaIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Phytohemagglutinin (PHA)-stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.
NCBI Gene ID #:
3690
NCBI Official Name:
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
NCBI Official Symbol:
ITGB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P05106
User NOte:
Optimal dilutions for each application to be determined by the researcher