Retinoblastoma / Rb Antibody [SPM353]

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ProSci
Product Code: PSI-33-784
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-784-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM353
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of human colon with Retinoblastoma antibody (clone SPM353). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human colon with Retinoblastoma antibody (clone SPM353). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Application Note:
WB: 0.5-1 ug/ml

IP: 1-2ug/500ug protein lysate

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Titering of the Retinoblastoma antibody may be required for optimal performance.
Background:
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.
NCBI Gene ID #:
5925
NCBI Official Name:
retinoblastoma 1
NCBI Official Symbol:
RB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle
Swissprot #:
P06400
User NOte:
Optimal dilutions for each application to be determined by the researcher