Calgranulin A + B Antibody [CAGB/426]
| Code | Size | Price |
|---|
| PSI-33-461-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CAGB/426
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.): 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the Calgranulin A + B antibody should be determined by the researcher.
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.): 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the Calgranulin A + B antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Background:
Recognizes a 12-14kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14); expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the Calgranulin A + B antibody.
NCBI Gene ID #:
6280
NCBI Official Name:
S100 calcium binding protein A9
NCBI Official Symbol:
S100A9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
PEG precipitation
Research Area:
Signal Transduction, Cancer
Swissprot #:
P06702
User NOte:
Optimal dilutions for each application to be determined by the researcher