CD45RO Antibody [190-2F2.5]

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ProSci
Product Code: PSI-33-301
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-301-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 190-2F2.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone 190-2F2.5). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone 190-2F2.5). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 1-2 ug/ml

Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.
Background:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.
NCBI Gene ID #:
5788
NCBI Official Name:
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol:
PTPRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P08575
User NOte:
Optimal dilutions for each application to be determined by the researcher