Thymidine Phosphorylase Antibody [P-GF.44C]

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ProSci
Product Code: PSI-33-768
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-768-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: P-GF.44C
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human placenta with Thymidine Phosphorylase antibody (clone P-GF.44C). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human placenta with Thymidine Phosphorylase antibody (clone P-GF.44C). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, PD-ECGF, TdRPase, TYMP, ECGF1
Application Note:
IP: 1-2ug/500ug lysate

IHC (FFPE): 1-2 ug/ml for 30 min at RT

WB: 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.
Background:
Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.
NCBI Gene ID #:
1890
NCBI Official Name:
Thymidine phosphorylase
NCBI Official Symbol:
TYMP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P19971
User NOte:
Optimal dilutions for each application to be determined by the researcher