p53 Antibody [TRP/816]

Product Code: PSI-33-855

Supplier: ProSci

Code	Size	Price

PSI-33-855-100ug

100ug

£765.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2a

Antibody Clonality: Monoclonal

Antibody Clone: TRP/816

Regulatory Status: RUO

Target Species: Human

Applications:

Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Shipping:

blue ice

Storage:

Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TP53 antibody (clone TRP/816). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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Documents

Further Information

Additional Names:

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Application Note:

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Optimal dilution of the TP53 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:

Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Buffer:

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration:

0.2 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Recombinant human protein was used as the immunogen for the TP53 antibody.

NCBI Gene ID #:

7157

NCBI Official Name:

tumor protein p53

NCBI Official Symbol:

TP53

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Purification:

Protein G affinity chromatography

Research Area:

Cancer,Cell Cycle,Signal Transduction,

Swissprot #:

P04637

User NOte:

Optimal dilutions for each application to be determined by the researcher