CD28 Antibody [204.12]

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ProSci
Product Code: PSI-33-494
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-494-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 204.12
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Porcine (pig)
  • Sheep
Applications:
  • Functional Study
  • Immunofluorescence (IF)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).
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Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).

Documents

Further Information

Additional Names:
CD4 Antibody [9H5A8] : CD4mut
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Functional Activity (Order SAF formulation)

Optimal dilution of the CD28 antibody should be determined by the researcher.
Background:
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
NCBI Gene ID #:
920
NCBI Official Name:
CD28 molecule
NCBI Official Symbol:
CD28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P10747
User NOte:
Optimal dilutions for each application to be determined by the researcher