Villin Antibody [VIL1/1314]

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ProSci
Product Code: PSI-33-717
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-717-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: VIL1/1314
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1314). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1314). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1314). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1314). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
VIL1, D2S1471, Villin, Villin 1, Villin-1, VIL
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

Western blot: 1-2 ug/ml

IHC (FFPE): 0.25-0.5 ug/ml for 30 min at RT

Titration of the Villin antibody may be required for optimal performance.
Background:
Recognizes a protein of ~93kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
NCBI Gene ID #:
7429
NCBI Official Name:
villin 1
NCBI Official Symbol:
VIL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Homeostasis
Swissprot #:
P09327
User NOte:
Optimal dilutions for each application to be determined by the researcher