IPO-38 Antibody [IPO-38]
Code | Size | Price |
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PSI-33-527-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: IPO-38
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Images
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Further Information
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the IPO-38 antibody should be determined by the researcher.
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the IPO-38 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Recognizes a protein of 14-16kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.
Physical State:
Liquid
Purification:
PEG precipitation
Research Area:
Cancer
Swissprot #:
Not Known
User NOte:
Optimal dilutions for each application to be determined by the researcher