Interferon alpha 2 Antibody [N27]

ProSci
Product Code: PSI-33-720
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-720-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: N27
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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SDS-PAGE Analysis of Purified, BSA-Free Interferon alpha 2 Antibody (clone N27). Confirmation of Integrity and Purity of the Antibody.

SDS-PAGE Analysis of Purified, BSA-Free Interferon alpha 2 Antibody (clone N27). Confirmation of Integrity and Purity of the Antibody.

Further Information

Additional Names:
Interferon alpha-2, Interferon alpha-A, IFN-alpha-2, IFNA, INFA2, IFNA2B, IFN-alphaA,
Application Note:
ELISA: order BSA/azide free format for coating

Blocking: order BSA/azide free format

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 0.5-1 ug/ml

Optimal dilution of the Interferon alpha 2 antibody should be determined by the researcher.
Background:
Recognizes a protein of 16-27kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified recombinant human IFNA2 was used as the immunogen for the Interferon alpha 2 antibody.
NCBI Gene ID #:
3440
NCBI Official Name:
interferon, alpha 2
NCBI Official Symbol:
IFNA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Chemokines & Cytokines
Swissprot #:
P01563
User NOte:
Optimal dilutions for each application to be determined by the researcher