Recombinant CFTR Antibody [CFTR/1775R]
| Code | Size | Price |
|---|
| PSI-33-787-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG, k
Antibody Clonality: Recombinant Antibody
Antibody Clone: CFTR/1775R
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR
Application Note:
IF: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant partial protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this recombinant CFTR antibody.
NCBI Gene ID #:
1080
NCBI Official Name:
cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
NCBI Official Symbol:
CFTR
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P13569
User NOte:
Optimal dilutions for each application to be determined by the researcher