Desmoglein 2 Antibody [8E5.]
| Code | Size | Price |
|---|
| PSI-33-655-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 8E5.
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.
Background:
Recognizes a protein of 165kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.
NCBI Gene ID #:
1829
NCBI Official Name:
desmoglein 2
NCBI Official Symbol:
DSG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q14126
User NOte:
Optimal dilutions for each application to be determined by the researcher