Interferon gamma Antibody [IFNG/466]

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ProSci
Product Code: PSI-33-577
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-577-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: IFNG/466
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human renal cell carcinoma with IFN gamma antibody (clone IFNG/466). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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SDS-PAGE Analysis of Purified, BSA-Free IFN gamma Antibody (clone IFNG/466). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human renal cell carcinoma with IFN gamma antibody (clone IFNG/466). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free IFN gamma Antibody (clone IFNG/466). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
IFG, IFIInterferon gamma, Immune interferon, IFN-gamma
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the IFN gamma antibody should be determined by the researcher.
Background:
Recognizes a protein of 20-25kDa, identified as human interferon. This mAb is specific to human IFNg and recognizes both recombinant and native human IFNg. T lymphocytes and NK cells mainly produce IFNg. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFNa/b (type I interferon).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant protein was used as the immunogen for the IFN gamma antibody.
NCBI Gene ID #:
3458
NCBI Official Name:
interferon, gamma
NCBI Official Symbol:
IFNG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P01579
User NOte:
Optimal dilutions for each application to be determined by the researcher