CD14 Antibody [LPSR/927]

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ProSci
Product Code: PSI-33-302
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-302-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: LPSR/927
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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SDS-PAGE Analysis of Purified, BSA-Free CD14 Antibody (clone LPSR/927). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free CD14 Antibody (clone LPSR/927). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 0.5-1 ug/ml





Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.
Background:
Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human CD14 recombinant protein was used as immunogen for this CD14 antibody.
NCBI Gene ID #:
929
NCBI Official Name:
CD14 molecule
NCBI Official Symbol:
CD14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology,Signal Transduction
Swissprot #:
P08571
User NOte:
Optimal dilutions for each application to be determined by the researcher