IgG Antibody [SPM556]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with IgA, IgM, IgE, or IgD, heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Purified human Ig Gamma Chain was used as the immunogen for this IgG antibody.

3500, 3501, 3502, 3503

immunoglobulin heavy constant gamma 1 (G1m marker), immunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant gamma 3 (G3m marker), immunoglobulin heavy constant gamma 4 (G4m marker)

IGHG1, IGHG2, IGHG3, IGHG4

homo sapiens

Liquid

Protein G affinity chromatography

Immunology

P01857, P01859, P01860, P01861

Optimal dilutions for each application to be determined by the researcher