IgG Heavy Chain Antibody

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ProSci
Product Code: PSI-33-811
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-811-100ug100ug£765.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

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IHC testing of human tonsil and IgG antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human tonsil and IgG antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

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Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

The optimal dilution of the IgG antibody for each application should be determined by the researcher.
Background:
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human Ig Gamma Chain was used as the immunogen for this IgG antibody.
NCBI Gene ID #:
3500
NCBI Official Name:
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol:
IGHG1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Immunology
Swissprot #:
P01857
User NOte:
Optimal dilutions for each application to be determined by the researcher