Recombinant IgG Heavy Chain Antibody [IG507R]

ProSci
Product Code: PSI-34-069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-069-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: IG507R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human tonsil with recombinant IgG antibody (clone IG507R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
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IHC testing of FFPE human tonsil with recombinant IgG antibody (clone IG507R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

The optimal dilution of the recombinant IgG antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.
NCBI Gene ID #:
3500
NCBI Official Name:
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol:
IGHG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Immunology
Swissprot #:
P01857
User NOte:
Optimal dilutions for each application to be determined by the researcher