MUC2 Antibody [SPM296]

MUC2, Intestinal mucin-2, Mucin-like protein, MUC-2, Mucin 2, intestinal/tracheal, Mucin 2, Mucin-2, MLP, SMUC

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the MUC2 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This mAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Amino acids KYPTTTPISTTTMVTPTPTPTGTQTPTTT from the human protein were used as the immunogen for this MUC2 antibody.

4583

mucin 2, oligomeric mucus/gel-forming

MUC2

Homo sapiens

Liquid

Protein G affinity chromatography

Cancer, Signal Transduction

Q02817

Optimal dilutions for each application to be determined by the researcher