Clatherin Light Chain Antibody [SPM174]

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ProSci
Product Code: PSI-33-667
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-667-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM174
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone SPM174). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.
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IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone SPM174). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.

Documents

Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.
Background:
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.
NCBI Gene ID #:
1211, 1212
NCBI Official Name:
clathrin light chain A, clathrin light chain B
NCBI Official Symbol:
CLTA, CLTB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P09496, P09497
User NOte:
Optimal dilutions for each application to be determined by the researcher