S100B Antibody [PS1B1-1]

Product Code: PSI-33-850

Supplier: ProSci

Code	Size	Price

PSI-33-850-100ug

100ug

£765.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2a, kappa

Antibody Clonality: Monoclonal

Antibody Clone: PS1B1-1

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)

Shipping:

blue ice

Storage:

Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 4

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100B antibody (clone PS1B1-1). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

2 / 4

3 / 4

4 / 4

Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).

❮❯

Documents

Further Information

Additional Names:

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

Application Note:

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistology (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Optimal dilution of the S100B antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Buffer:

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration:

0.2 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.

NCBI Gene ID #:

6285

NCBI Official Name:

S100 calcium binding protein B

NCBI Official Symbol:

S100B

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Purification:

Protein G affinity chromatography

Research Area:

Cell Cycle,Signal Transduction

Swissprot #:

P04271

User NOte:

Optimal dilutions for each application to be determined by the researcher