S100A4 Antibody [S100A4/1481]

Contact us
ProSci
Product Code: PSI-33-780
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-780-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: S100A4/1481
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 6
IHC testing of FFPE human placenta with FSP1 antibody (clone S100A4/1481). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.
2 / 6
3 / 6
4 / 6
IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1481, green) and Reddot nuclear stain (red).
5 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100A4 antibody (clone S100A4/1481). These results demonstrate the foremost specificity of the S100A4/1481 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
6 / 6
Western blot testing of human HeLa cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.
❮❯

IHC testing of FFPE human placenta with FSP1 antibody (clone S100A4/1481). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.
IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1481, green) and Reddot nuclear stain (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100A4 antibody (clone S100A4/1481). These results demonstrate the foremost specificity of the S100A4/1481 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Western blot testing of human HeLa cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

Documents

Further Information

Additional Names:
S100A4, S100 calcium binding protein A4, 18A2, 42A, CAPL, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, malignant transformation suppression 1
Application Note:
Western blot: 0.5-1 ug/ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT

The optimal dilution of the FSP1 antibody for each application should be determined by the researcher.
Background:
S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. FSP1 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. FSP1 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant S100A4/FSP1 protein was used as the immunogen for this FSP1 antibody.
NCBI Gene ID #:
6275
NCBI Official Name:
S100 calcium binding protein A4
NCBI Official Symbol:
S100A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P26447
User NOte:
Optimal dilutions for each application to be determined by the researcher