S100A4 Antibody [S100A4/1482]

ProSci
Product Code: PSI-33-781
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-781-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: S100A4/1482
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.
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Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

Further Information

Additional Names:
S100A4, S100 calcium binding protein A4, 18A2, 42A, CAPL, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, malignant transformation suppression 1
Application Note:
Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

The optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
Background:
S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
NCBI Gene ID #:
6275
NCBI Official Name:
S100 calcium binding protein A4
NCBI Official Symbol:
S100A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P26447
User NOte:
Optimal dilutions for each application to be determined by the researcher