SHBG Antibody [TTEB3]

ProSci
Product Code: PSI-33-984
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-984-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: TTEB3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human testicular carcinoma with SHBG antibody (clone TTEB3)

IHC testing of FFPE human testicular carcinoma with SHBG antibody (clone TTEB3)

Further Information

Additional Names:
Sex hormone-binding globulin, ABP, SBP, TEBG
Application Note:
Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the SHBG antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Sex Hormone Binding Globulin, also called Androgen Binding Protein (ABP) and Testosterone-Estradiol-Binding Globulin (TEBG), functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full-length human protein was used as the immunogen for the SHBG antibody.
NCBI Gene ID #:
6462
NCBI Official Name:
sex hormone-binding globulin
NCBI Official Symbol:
SHBG
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity
Swissprot #:
P04278
User NOte:
Optimal dilutions for each application to be determined by the researcher