SOX2 Antibody [SOX2/1791]
| Code | Size | Price |
|---|
| PSI-33-743-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SOX2/1791
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2
Application Note:
ELISA: 2-4 ug/ml; order BSA-free formulation for coating
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the SOX-2 antibody should be determined by the researcher.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the SOX-2 antibody should be determined by the researcher.
Background:
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 176-305 of the human protein were used as the immunogen for the SOX-2 antibody.
NCBI Gene ID #:
6657
NCBI Official Name:
SRY (sex determining region Y)-box 2
NCBI Official Symbol:
SOX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Homeostasis,Stem Cell
Swissprot #:
P48431
User NOte:
Optimal dilutions for each application to be determined by the researcher