SPTBN2 Antibody [SPTBN2/1584]

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ProSci
Product Code: PSI-33-753
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-753-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: SPTBN2/1584
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1584). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1584). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

WB: 0.5-1 ug/ml



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
Background:
Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alphaII (fodrin alpha) and spectrin betaI (fodrin beta) are present in other somatic cells. SPTBN2/Spectrin betaIII is highly homologous to both spectrin betaI and spectrin betaII. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
NCBI Gene ID #:
6712
NCBI Official Name:
spectrin beta, non-erythrocytic 2
NCBI Official Symbol:
SPTBN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
O15020
User NOte:
Optimal dilutions for each application to be determined by the researcher