H-2Db Antibody [28-14-8]

MHC Class I

H-2Db Antibody [28-14-8] for use in flow cytometry, immunohistochemistry, immunoprecipitation, CMCD, and blocking assays.

The ?classical? MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.

Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

0.5 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

C3H.SW mouse splenocytes

14964

histocompatibility 2, D region locus 1

H2-D1

Mus musculus

Liquid

P01899

Immunology

H-2Db

P01899

Optimal dilutions for each application to be determined by the researcher.