H-2Kd/H-2Dd Antibody [34-1-2S]

MHC Class I

H-2Kd/H-2Dd Antibody [34-1-2S] for use in flow cytometry, immunocytochemistry, electron microscopy, immunoprecipitation, CMCD, blocking, and adhesion assays.

The ?classical? MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 34-1-2S binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Kb.

Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

0.5 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

BDF1 mouse splenocytes

14964/14972

histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region

H2-D1; H2-K1

Mus musculus

Liquid

Immunology

H-2Kd/H-2Dd

Optimal dilutions for each application to be determined by the researcher.