Caspase-2 Antibody [SB123a]

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ProSci
Product Code: PSI-99-808
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-808-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: [SB123a]
Regulatory Status: RUO
Target Species: Human
Applications:
  • Electron microscopy
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Total cell lysates from Jurkat cells were incubated with Rabbit Anti-Human DR5-UNLB , resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-2-UNLB (Cat. No. 99-808). Proteins were visualized using Goat Anti-Rat IgM-HRP secondary antibody and chemiluminescent detection.
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Total cell lysates from Jurkat cells were incubated with Rabbit Anti-Human DR5-UNLB , resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-2-UNLB (Cat. No. 99-808). Proteins were visualized using Goat Anti-Rat IgM-HRP secondary antibody and chemiluminescent detection.

Documents

Further Information

Additional Names:
Nedd2, ICH-1, CASP2
Application Note:
Caspase-2 Antibody [SB123a] for use in western blotting assays.
Background:
The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-2 is an initiator caspase that is prevalent in both the cytosol and nucleus in many cell types. During embryonic development it is expressed at relatively high levels in the central nervous system, liver, kidney, and lungs and is prevalent in numerous tissues in adults. Studies have shown that caspase-2 becomes activated in response to several lethal stimuli including DNA damage, ER stress, and death receptor stimulation and further evidence has suggested a role in reactive oxygen species-induced cell death. The SB123a monoclonal antibody detects bands at ~40 and ~48 kDa corresponding to cleaved and uncleaved forms of caspase-2, respectively.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
1.0 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified native human caspase-2
NCBI Gene ID #:
835
NCBI Official Name:
caspase 2, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P42575
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Caspase-2
Swissprot #:
P42575
User NOte:
Optimal dilutions for each application to be determined by the researcher.