Caspase-8 Antibody [SB125a]

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ProSci
Product Code: PSI-99-811
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-811-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: [SB125a]
Regulatory Status: RUO
Target Species: Human
Applications:
  • Electron microscopy
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Total cell lysates from Jurkat cells were incubated with STS, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-8-UNLB (Cat. No. 99-811). Proteins were visualized using Goat Anti-Rat IgM-HRP secondary antibody and chemiluminescent detection.
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Total cell lysates from Jurkat cells were incubated with STS, resolved by electrophoresis, transferred to PVDF membrane, and probed with Rat Anti-Human Caspase-8-UNLB (Cat. No. 99-811). Proteins were visualized using Goat Anti-Rat IgM-HRP secondary antibody and chemiluminescent detection.

Documents

Further Information

Additional Names:
MACH, FLICE, Mch5
Application Note:
Caspase-8 Antibody [SB125a] for use in western blotting assays.
Background:
The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-8 is a cytosolic protease that is capable of processing/activating all known caspases, indicating its presence early in the apoptotic cascade. It mediates cell death induction by receptors of the tumor necrosis factor (TNF) and nerve growth factor (NGF) family to eliminate injured cells and maintain leukocyte homeostasis. Moreover, studies have suggested it regulates Fas-induced cytotoxicity and may have a non-apoptotic role in macrophage differentiation. The SB125a monoclonal antibody detects a band at ~55kD corresponding to the full length form of caspase-8.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
1.0 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified native human caspase-8
NCBI Gene ID #:
841
NCBI Official Name:
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q14790
Research Area:
Apoptosis
SPECIFICITY:
Caspase-8
Swissprot #:
Q14790
User NOte:
Optimal dilutions for each application to be determined by the researcher.