CD3e Antibody [C3e/1308]

ProSci
Product Code: PSI-33-654
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-654-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: C3e/1308
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
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IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Further Information

Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Application Note:
WB: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Optimal dilution of the CD3e antibody should be determined by the researcher.
Background:
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
NCBI Gene ID #:
916
NCBI Official Name:
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol:
CD3E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P07766
User NOte:
Optimal dilutions for each application to be determined by the researcher