TIM-3 Antibody [1H4]
| Code | Size | Price |
|---|
| PSI-RF16104-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-RF16104-0.1mg | 0.1mg | £515.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 1H4
Regulatory Status: RUO
Target Species: Human
Application: Immunocytochemistry (ICC)
Shipping:
blue ice
Storage:
TIM-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3
Application Note:
TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Flow Cytometry start at 0.1 μg/ml.
Background:
The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Background References:
- Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
- Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187?94.
- Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501?10.
Buffer:
TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIM-3 antibody was raised against the extracellular domain of human TIM-3
NCBI Gene ID #:
84868
NCBI Official Name:
hepatitis A virus cellular receptor 2
NCBI Official Symbol:
HAVCR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116171
Protein GI Number:
84868
Purification:
TIM-3 Antibody is supplied as protein A purified IgG1.
Research Area:
Cell Cycle,Cancer,Immunology
Swissprot #:
Q8TDQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.