TIM-3 Antibody [2A6]

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ProSci
Product Code: PSI-RF16106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-RF16106-0.02mg0.02mg£150.00
Quantity:
PSI-RF16106-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 2A6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
TIM-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of TIM-3 in overexpressing HEK293 cells TIM-3 antibody at (A) 0.25, (B) 0.5, and (C) 1 μg/ml
2 / 6
Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL. <br><br>Green: TIM-3 Antibody [2A6] (RF16106) <br> Blue: DAPI staining
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Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL. <br><br>Green: TIM-3 Antibody [2A6] (RF16106) <br> Blue: DAPI staining
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Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.
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Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.
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Western blot analysis of TIM-3 in overexpressing HEK293 cells TIM-3 antibody at (A) 0.25, (B) 0.5, and (C) 1 μg/ml
Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL. <br><br>Green: TIM-3 Antibody [2A6] (RF16106) <br> Blue: DAPI staining
Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL. <br><br>Green: TIM-3 Antibody [2A6] (RF16106) <br> Blue: DAPI staining
Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.
Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.

Documents

Further Information

Additional Names:
TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3
Application Note:
TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Western blot start at 1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml.
Background:
The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Background References:
  • Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
  • Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187?94.
  • Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501?10.
Buffer:
TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIM-3 antibody was raised against the extracellular domain of human TIM-3
NCBI Gene ID #:
84868
NCBI Official Name:
hepatitis A virus cellular receptor 2
NCBI Official Symbol:
HAVCR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33 kDa

Observed: 37 kDa
Protein Accession #:
NP_116171
Protein GI Number:
84868
Purification:
TIM-3 Antibody is supplied as protein A purified IgG1.
Research Area:
Cell Cycle,Cancer,Immunology
Swissprot #:
Q8TDQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.