Alpha 1 Antitrypsin Antibody [AAT/1378]

ProSci
Product Code: PSI-33-598
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-598-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: AAT/1378
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
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IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

Further Information

Additional Names:
Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, Short peptide from AAT, SPAAT, SERPINA1, AAT, PI
Application Note:
Western blot: 0.5-1 ug/ml

IHC (Paraffin): 0.5-1 ug/ml

IF: 0.5-1 ug/ml

Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml

The stated application concentrations are suggested starting amounts. Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
The immunohistochemical staining of Alpha 1 Antitrypsin (AAT) is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.
NCBI Gene ID #:
5265
NCBI Official Name:
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol:
SERPINA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity
Research Area:
Cancer,Cell Cycle,Obesity
Swissprot #:
P01009
User NOte:
Optimal dilutions for each application to be determined by the researcher