VISTA Antibody
| Code | Size | Price |
|---|
| PSI-8695-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8695-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54
Application Note:
VISTA antibody can be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. For Immunocytochemistry start at 1 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
Background References:
- Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
- Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
Buffer:
VISTA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA.
The immunogen is located within the last 60 amino acids of VISTA.
The immunogen is located within the last 60 amino acids of VISTA.
NCBI Gene ID #:
64115
NCBI Official Name:
VISTA molecule
NCBI Official Symbol:
VSIR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071436
Protein GI Number:
64115
Purification:
VISTA Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q9H7M9
User NOte:
Optimal dilutions for each application to be determined by the researcher.