Mitochondrial Antibody [MTL56]
| Code | Size | Price |
|---|
| PSI-33-878-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MTL56
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
IF: 0.5-1 ug/ml
Western blot: 1-2 ug/ml
Titering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Prediluted format : incubate for 30 min at RT (2)
IF: 0.5-1 ug/ml
Western blot: 1-2 ug/ml
Titering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
The mitochondrial marker antibody is specific for an antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and as a mitochondrial marker in subcellular fractions.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human mitochondria were used as the immunogen for this Mitochondrial antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity
User NOte:
Optimal dilutions for each application to be determined by the researcher