Monocyte/Macrophage Antibody [KUL01]

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ProSci
Product Code: PSI-99-329
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-329-0.5mg0.5mg£597.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [KUL01]
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Flow Cytometry
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Normal chick cochlea was stained with phalloidin conjugated to AF546 and Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by an AF488 secondary antibody. Image from Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. Figure 1(b)
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Frozen IBV infected chicken tracheal explant section were stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) and FITC conjugated anti-IBV followed by a secondary antibody and Hoechst 33342. Image from Reddy VR, Trus I, Desmarets LM, Li Y, Theuns S, Nauwynck HJ. Productive replication of nephropathogenic infectious bronchitis virus in peripheral blood monocytic cells, a strategy for viral dissemination and kidney infection in chickens. Vet Res. 2016;47:70. Figure 1(b)
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Paraffin embedded low pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(d)
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Paraffin embedded high pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(e)
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Frozen vIBDV infected bursal tissue section was stained with Mouse Anti-Chicken Monocyte/ Macrophage-UNLB (Cat. No. 99-329) followed by a secondary reagents and DAB. Image from Rauf A, Khatri M, Murgia MV, Jung K, Saif YM. Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virus. Vet Res. 2011;42:85. Figure 4[A(d)]
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Normal chick cochlea was stained with phalloidin conjugated to AF546 and Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by an AF488 secondary antibody. Image from Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. Figure 1(b)
Frozen IBV infected chicken tracheal explant section were stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) and FITC conjugated anti-IBV followed by a secondary antibody and Hoechst 33342. Image from Reddy VR, Trus I, Desmarets LM, Li Y, Theuns S, Nauwynck HJ. Productive replication of nephropathogenic infectious bronchitis virus in peripheral blood monocytic cells, a strategy for viral dissemination and kidney infection in chickens. Vet Res. 2016;47:70. Figure 1(b)
Paraffin embedded low pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(d)
Paraffin embedded high pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken Monocyte/Macrophage-UNLB (Cat. No. 99-329) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(e)
Frozen vIBDV infected bursal tissue section was stained with Mouse Anti-Chicken Monocyte/ Macrophage-UNLB (Cat. No. 99-329) followed by a secondary reagents and DAB. Image from Rauf A, Khatri M, Murgia MV, Jung K, Saif YM. Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virus. Vet Res. 2011;42:85. Figure 4[A(d)]

Documents

Further Information

Application Note:
Monocyte/Macrophage Antibody [KUL01] for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
Background:
The monoclonal antibody KUL01 is useful in the study of the development, distribution, function, and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1+) or T (CD3+) lymphocytes.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken peripheral blood mononuclear cell leukocytes
Physical State:
Liquid
Research Area:
Immunology
SPECIFICITY:
Monocyte/Macrophage
User NOte:
Optimal dilutions for each application to be determined by the researcher.