TCRab/Vb2 Antibody [TCR-3] (PE)

T3/TCR complex, TCR alpha/beta

TCRab/Vb2 Antibody [TCR-3] for use in flow cytometry assays.

The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of alphabeta T cells appear in the thymus subsequent to the appearance of gammadelta T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- subpopulations. The TCR-3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR-3+] cells in the blood and spleen express relatively high levels of the TCR-3/CD3 receptor complex and are ?single positive? with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (~ 80% CD4+ vs ~ 20% CD8+).

Supplied in PBS/NaN3 and a stabilizing agent

0.1 mg/mL

PE

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells

Liquid

Immunology

TCRalphabeta/Vbeta2

Optimal dilutions for each application to be determined by the researcher.